![]() Because the conjugation of dye to proteins in the CLEARLY Stained Protein Ladder affects migration of the highest molecular weight proteins in the ladder, it is not recommended as a reference for precise molecular weight calculations. The markers have been carefully prepared under sterile conditions and stored in appropriate additives to ensure long term stability.Īllows monitoring protein migration during electrophoresis. Use the CLEARLY Stained Protein Ladder to easily monitor the efficiency of protein transfer to a Western blot. The three colour identification in ladder helps in easy orientation of the molecular sizes of the protein bands. All the bands are bonded to a blue chromophore except for 75 kDa (red chromophore and 25 kDa (green chromophore) 7.4.6 Immunochemical Analysis (Western Blot and ELISA) Antibodies. Ladder should show 10 prestained protein bands during SDS PAG electrophoresis.īioLit Prestained High Range 1 Tri-Coloured Protein Ladder is a Ready-To-Use solution of carefully selected 10 stained proteins within a wide range of molecular mass (10-180 kDa). The Bullseye Pre-stained Protein Ladder contains 10 prestained proteins, covering a wide range of molecular weights from 10 to 180kDa. For SDS-PAGE, choose from prestained and unstained, natural or recombinant protein ladders for broad, high, and low molecular weight ranges. IMDG Identification :Not Regulated for Transport (Non-Haz) Bio-Rad protein ladders have been formulated and combined for use as molecular weight markers in a variety of applications including SDS-PAGE, IEF, 2-D PAGE, and western blotting. In addition, these colored markers show evidence for the transfer of protein bands on the membrane in western blotting technique. It allows continuous monitoring of protein separations. The Prestained Protein Ladder is ideally suited for use in SDS-PAGE and western blotting applications. 27839 PreStained High Range 1 Tri-Coloured Protein Ladder: 10-180kDa The apparent molecular weight of the Prestained Protein Ladder was determined on Invitrogen Novex 10-20 Tris-glycine SDS PAGE gels (1,2) by comparison to the Protein Ladder (NEB P7703).
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